Single-particle Analytics, Real-time Dynamics
CLiC consumable fits on
the microscope stage
Pneumatic mechanism confines molecules/particles/cells in the microwells
Microwell array shows
single-particles in solution
Convex Lens-induced Confinement (CLiC) is ScopeSys' proprietary analytics platform for observing biomolecules under biologically relevant conditions. Instead of relying on population averages, CLiC measures individual particles and molecules one at a time, generating rich, correlated datasets that reveal how complex therapeutic systems behave.
For genomic medicines, CLiC is especially powerful because researchers can move beyond basic size and encapsulation measurements to understand particle-to-particle heterogeneity, cargo loading, molecular organization, and dynamic interactions that influence important drug properties.
Sample is introduced into a microfluidic flow cell containing patterned confinement microwells.
A pneumatic mechanism gently deflects the top glass layer, creating a thin observation volume that restricts out-of-plane diffusion.
Particles drift naturally in solution enabling native measurements of motion, structure, and interaction behavior.
Unique imaging sequences capture fluorescence intensity and FRET to generate formulation-specific fingerprints.
Algorithms convert thousands of single-particle trajectories into precise quantitative distributions, uncovering hidden subpopulations.
CLiC generates correlated single-particle measurements that help researchers understand not only what is in a formulation, but how each particle is built and how it behaves.
Track individual motion to estimate hydrodynamic diameter and mobility.
Reveal subpopulations that differ in size, loading, and molecular organization.
Differentiate empty and loaded particles; estimate payload copy number using single-molecule calibration.
Determine whether each particle contains one or multiple cargo species.
Use multicolor fluorescence and FRET to probe molecular arrangement within individual nanoparticles.
Measure particle-particle and particle-environment interactions in real time, including LNP fusion and aggregation.
Monitor how pH, ionic strength and buffer exchange affect stability, aggregation and fusogenicity.
See the particle-level differences that averages miss, measure real-time dynamic behavior and connect them to development decisions.
Resolves full distributions and rare subpopulations beyond single bulk averages
Highlights particle-level differences that can drive potency and safety
Links size, cargo loading, molecular organization and dynamics on the same particle
Shows how particle qualities relate to function all in one assay
Observes freely diffusing particles in solution and in real time under changing conditions
Captures fusion, aggregation and stability without immobilization artifacts
Creates particle-resolved fingerprints across batches, processes and manufacturing conditions
Supports formulation selection, process optimization, and future quality control
Produces high-resolution, multiparametric datasets that are ready for advanced analytics
Enables predictive models for drug design, optimization and process understanding
Discuss your LNP analytics challenge with us
Contact Us