Publications
ScopeSys is a spin-off company from the laboratory of Dr. Sabrina Leslie at UBC. The following publications are the output of this lab, some of which are collaborative with ScopeSys through sponsored research and collaborative research agreements. These publications are a great introduction to the single-molecule and single-cell imaging platform which ScopeSys is commercializing.
Single-particle RNA-LNP analytics
Simultaneous, single-particle measurements of size and loading give new insights into the structure of drug-delivery nanoparticles
A. Kamanzi, Y. Gu, R. Tahvildari, Z. Friedenberger, X. Zhu, R. Berti, M. Kurylowicz, D. Witzigmann, J. Kulkarni, J. Leung, John Andersson, Andreas Dahlin, Fredrik Hook, Mark Sutton, Pieter R. Cullis, and Sabrina R. Leslie*
Single-molecule DNA-interaction analytics
Non-equilibrium structural dynamics of supercoiled DNA plasmids exhibit asymmetrical relaxation.
C. Shaheen, C. Hastie, K. Metera, S. Scott, Z. Zhang, S. Chen, G. Gu, L. Weber, B. Munsky, D. Levens, C. Benham, and Sabrina R. Leslie*
Single-molecule visualization of the effects of ionic strength and crowding on structure-mediated interactions in supercoiled DNA molecules
S. Scott, C. Shaheen, B. McGuiness, K. Metera, F. Kouzine, D. Levens, C. J. Benham, Sabrina R. Leslie*
Visualizing structure-mediated interactions in supercoiled DNA molecules
S. Scott, Zhi Ming Xu, Fedor Kouzine, Daniel J Berard, Cynthia Shaheen, Barbara Gravel, Laura Saunders, Alexander Hofkirchner, Catherine Leroux, Jill Laurin, David Levens, Craig J Benham, Sabrina R Leslie*
Single-molecule protein-interaction analytics
Probing inhomogeneous diffusion in the microenvironments of phase-separated polymers under confinement”
M. Shayegan, R. Tahvildari, L. Kisley, K. Metera, S. W. Michnick*, Sabrina R. Leslie*
Journal of the American Chemical Society. (2019), 141, 19, 7751-7757
Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear Zero Mode Waveguides (ZMW) and Convex Lens-induced Confinement (CLiC)
M. W. Elting, S. R. Leslie, L. S. Churchman, J. Korlach, C. McFaul, J. S. Leith, M. J. Levene, A. E. Cohen, J. A. Spudich
Single-cell analytics
Single-cell analysis for drug development using Convex Lens-Induced Confinement (CLiC) imaging
K. Thiombane, N. Coutin, D. J. Berard, R. Tahvildari, Sabrina R. Leslie*, C. Nislow*
Parallelized Cytoindentation Using Convex Micropatterned Surfaces
B. Jia, T.L. Wee, D. Berard, A. Mallik, D. Juncker, C. M. Brown*, Sabrina R. Leslie*
Development of a Platform for Single-Cell Genomics Using Convex Lens-Induced Confinement
S. Mahshid, M. J. Ahamed, D. Berard, S. Amin, R. Sladek, Sabrina R. Leslie*, W. Reisner*
CLiC microscopy and instrumentation development
Miniaturized flow cell with pneumatically-actuated vertical nanoconfinement for single-molecule imaging and manipulation
D. J. Berard, Sabrina R. Leslie*
Formatting and Ligating Biopolymers using Adjustable Nanoconfinement
D. Berard, M. Shayegan, F. Michaud, G. Henkin, S. Scott, Sabrina R. Leslie*
Manipulating and visualizing molecular interactions in customized nanoscale spaces
G. Henkin, D. Berard, F. Stabile, M. Shayegan, J. S. Leith, Sabrina R. Leslie*
Open-frame System for Single–Molecule Microscopy
A. Arsenault, J. S. Leith, G. Henkin, C. McFaul, M. Tarling, R. Talbot, D. Berard, F. Michaud, S. Scott, Sabrina R. Leslie*
Precision Platform for Convex Lens-Induced Confinement Microscopy
D. Berard, C. McFaul, J. S. Leith, A. Arsenault, F. Michaud, Sabrina R. Leslie*
Convex Lens-induced Confinement for Imaging Single Molecules
Sabrina R. Leslie, A. P. Fields, A. E. Cohen
Investigations of DNA polymers in confinement
Rotation-induced Macromolecular Spooling DNA
T. Shendruk, D. Sean, D. Berard, J. Wolf, J. Dragoman, S. Battat, G. Slater, Sabrina R. Leslie*
Free energy of a polymer in slit-like confinement from the Odijk to the bulk regime
J. S. Leith, A. Kamanzi, D. Sean, D. Berard, A. Guthrie, C.M.J. McFaul, G. Slater, H. de Haan, Sabrina R. Leslie*
Continuous Confinement Fluidics: Getting Lots of Molecules in Small Spaces
J. Ahamed, S. Mahshid, D. Berard, F. Michaud, R. Sladek, W. Reisner, Sabrina R. Leslie*
Convex lens-induced nanoscale templating
D. Berard, F. Michaud, S. Mahshid, M. J. Ahamed, C. McFaul, J. S. Leith, P. Berube, R. Sladek, W. Reisner, Sabrina R. Leslie*
Review and perspective articles
Single-molecule imaging of the biophysics of molecular interactions in cell-like conditions, without tethers, and with precision and control
Sabrina R. Leslie et al
In honor of W. E. Moerner: Confining molecules for single-molecule spectroscopy
A. E. Cohen, A. P. Fields, J. H. Hou, S. R. Leslie, M. J. Shon